此論文利用光介電泳力混合微球與樣本液，加速以微球為載體之蛋白質免疫檢測法之反應速率，減少反應時間。
根據文獻資料，微小化之蛋白質免疫檢測法，如蛋白質免疫檢測微陣列晶片，其理論反應時間很短。但是，當樣本液之濃度極低時，其樣本之擴散效應反而減緩了蛋白質之間的反應。實際的蛋白質免疫檢測微陣列檢測法反應時間變得非常長。擴散效應對反應速率造的影響在低濃度之樣本下更為明顯。
在本論文中，使用微球為載體之蛋白質免疫檢測法，以減少樣本液反應之擴散距離，並增加蛋白質間之反應面積。並利用光學介電泳力操縱微球，將微球與樣本液充分混合，以增加兩者之間之碰撞機率。
實驗中，表面處理覆蓋streptavidin之微球，利用光學介電泳力之操縱，可自由地在反應晶片內移動，增加與液體中之生物素結合之速率。
最後驗證經由光學介電泳力之混合，可增加微球蛋白質免疫檢測法之速率，並可縮短反應時間，為無攪拌狀態之三分之一。且可偵測之最低反應濃度為100nM。

This study demonstrated a method for accelerating the reaction of the micro-bead protein immunoassay via mixing of the beads with the sample.
According to the previous studies, when the scale of the protein immunoassay is small, such as micro-spot protein microarray, the theoretical reaction rate of the protein immunoassay microarray is fast. But when the concentration of the sample is very low, the reaction rate becomes very slow and it takes a long time to equilibrium the reaction. The effect of diffusion is very significant especially in low concentration and low volume.
In this study, the micro-bead protein immunoassay was used to reduce the diffusion distance and increase the reaction surface. Manipulation of the beads by optical dielectrophoretic force was to mix the beads with the sample fluid. Mixing increased the probability of collision between the beads and protein.
The streptavidin-coated beads were manipulated by the dielectrophoretic force induced by the light generated from the liquid crystal display (LCD) projector. The beads traveled through the biotin solution in the chamber on the chip. The movement of beads largely increased the collision probability between streptavidin on the beads and biotin in the fluids. Mixing was proved to increase the reaction rate of the micro-bead protein immunoassay via the manipulation by optical dielectrophoretic force. In this thesis, the time to reach equilibrium has been reduced to one third of that of the static incubation condition. The fluorescence intensity has also been increased to three times stronger than that of the condition of non-mixing. The limitation of detection of the mixing chip can be 100nM.

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