cDNA微陣列系統為一套可用來觀察大量基因表現之工具，屬於基因晶片中的一支，其硬體包含了點印系統及掃瞄系統兩大部份。約可同時觀察6000~10000個基因，在研究細胞生理及臨床診斷上是一個相當有力的技術。
本篇文章分成兩大部份，一部份是cDNA微陣列系統的探討，其中包含點印環境的探討、雜合條件的改變、不同的清洗方法及玻片點印完之後處理的改變，另一部份則是探討，經輻射照射後MRC5CVI細胞之基因表現。在系統的探討部份，我們想藉由雜合條件的改變，觀察是否能得到更好的雜合效率。並且藉由加入額外的清洗步驟、UV-crosslinking及不同的清洗方法，觀察在何種組合之下，我們可以得到最好的訊雜比值。而在輻射照射方面，本篇論文最主要之目的是，利用微陣列系統來分析MRC5CVI照射輻射前後的基因表現差異。此結果將用來當作，未來要比較正常和運動失調及微血管擴張症細胞，在相同物理劑量及相同存活劑量照射下，基因表現圖譜的差異之重要參考資料。

參考文獻
1.Biochips http://biotech.nature.com
2.Pease, A., Solas, D., Sullivan, Light-generated oligonucleotide arrays for rapid DNA sequence analysis. PANS 24:5022-6,1994
3.Brown PO, Botstein D, Exploring the new world of the genome with DNA microarray. Suppl Nat Genet 21:33-37,1999
4.Schena M, Shalon D, Heller R, Chai A, Brown PO, Davis RW, Parallel human genome analysis : Microarray-based expression monitoring of 1000 genes. Proc. Natl. Acad. Sci. USA. 93:10614-10619,1996
5.Christine E. Canman, Michael B. Kastan, Induction of apoptosis by tumor suppressor genes and oncogenes. Cancer Biology 6:17-25,1995
6.Debouck C. Goodfellow PN, DNA microarrays in drug discovery and development. Nature gene. 21:48-50,1999
7.Tanaka TS, Minoru SHK, Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray. PNAS. 97:9127-9132,2000
8.Ji-Hong Hong, Richard A.Gatti, Yong K.Huo, Chi-Shiun Chiang, Willian H.McBride G2/M-Phase Arrest and Release in Ataxia Telangiectasia and Normal Cells after Exposure to Ionizing Radiation. Radiation Researc. 140.17-23,1994
9. Stankovic Tatiana, Stewar Grant S.t, Fegan Christopher, Biggs Paul, James Last James, Byrd Philip J., Keenan Russell D., Moss Paul A. H., and Taylor Alexander M. R. Ataxia telangiectasia mutated–deficient B-cell chronic lymphocytic leukemia occurs in pregerminal center cells and results in defective damage response and unrepaired chromosome damage. BLOOD 99,2002
10. Zhang Yin, Alexandre Dimtchev, Anatoly Dritschilo, and Mira Jung
Ionizing Radiation-induced Apoptosis in Ataxia-Telangiectasia
Fibroblasts. Biological Chemistry 276:28842-28848,2001
11.Shackelford, Cynthia L. Innes, Stella O. Sieber, Alexandra N. Heinloth, Steven A. Leadon, and Richard S. Paules The Ataxia telangiectasia Gene Product Is Required for Oxidative Stress-induced G1 and G2 Checkpoint Function in Human Fibroblasts. Biological Chemistry 276:21951-21959,2001
12.Ste´phane Vispe´ and Masahiko S. Satoh DNA Repair Patch-mediated Double Strand DNA Break Formation in Human Cells. Biological Chemistry 275:27386-27392,2000
13.Thomas M. Houts Improved 2-Color “Exponential” Normalization For Microarray Analyses Employing Cyanine Dyes.
14.George C. Tseng, Min-Kyu Oh, Lars Rohlin Issues in cDNA microarray analysis:quality filtering, channel normalization, models of variations and assessment of gene effects. Nucleic Acids Research 29:2549-2557,2001
15.Yee Hwa Yang, Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. Nucleic Acids Research 30,2002
16.Callow,M.J., Dudoit, S., Gong,E.L., Speed,T.P. microarray expression profiling identifies genes with altered expression in HDL-deficient mice. Genome Res. 10:2022-2029,2000
17.Ihaka,R. and Gentleman,R. A language for data analysis and graphics. J. Comput. Graph. Statist., 5:299-341,1996
18.The Chipping forecast. Nature Genet. 21,1999
19. Virginia Goss Tusher, Robert Tibshirani, and Gilbert Chu Significance analysis of microarrays applied to the ionizing radiation response. PANS 98: 5116-5121,2001