直至今日，如何有效的幫助受損腦神經的再生，在醫學上仍然是個很大的難題。而導致神經無法有效率再生的情況主要歸因於抑制性的環境以及自身較差的生長能力。若能更加了解皮質神經細胞再生的機制將會對神經受損後的臨床治療有非常大的助益。在本篇論文中，我發現到WNT3A的表現量在懷孕18天胚鼠的大腦皮質神經細胞的再生過程中有上升的情形。而在隨後的實驗中，我們也證明將WNT3A加入受損的皮質神經中可以有效的幫助其再生。另外我們也找到三個WNT3A可能的標的基因，分別是BDNF、KLF7以及GAP43。此外，3D腦組織切片培養的方法也被利用於模擬腦中的生長環境，同時也用於評估WNT3A在腦切片組織的再生過程中所扮演的角色。

Regeneration of injured brain neurons remains to be a challenging medical issue. The inability of injured neurons to regenerate is mainly attributed to an inhibitory environment and poor intrinsic growth capacity. Better understanding of the neuronal regeneration mechanism would help the development of clinical treatment for brain injury. In this thesis, I have found that the expression level of WNT3A was increased during regeneration of injured embryonic day 18 cortical neurons. Treating injured cortical neurons with WNT3A effectively promotes neuronal regeneration. Three candidate target genes of WNT3A signaling, brain-derived neurotrophic factor, Krüppel-like factor 7 and growth associated protein 43, were identified. In addition, 3D brain slice culture was used to mimic the in vivo environment of brain and evaluate the role of WNT3A in neuronal regeneration.

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