過去建立神經網路的研究中，通常會利用微壓印技術在培養基板轉印出特定分子圖案，促使投入的神經細胞貼附到培養基板的特定分子圖案區域，沿著圖案長成幾何神經網路。本研究中建立了一個方法學，試著用和微壓印技術不同系統的方法製成更適合幾何神經網路形成的裝置。實驗中以光學顯影技術製作微米等級的模仁翻製出具有篩板功能的PDMS模板。此模板頂部具有64個圓孔，以8 × 8陣列形式排列，底部則為7 × 7格形網狀溝槽，上下相通。透過加壓手法將PDMS模板貼附到玻片，加入poly-L-lysine (PLL)使其流入孔洞，在玻片上建立較高解析度的格形網狀圖案。投入細胞時利用離心力縮短細胞掉落時間，避免細胞聚集成團降低具有神經細胞的節點數百分比。PDMS模板可侷限神經細胞掉落在PLL圖案中的節點上，並沿著節點連接線生長形成神經網路，此外我們針對低密度神經網路培養調整培養程序。藉由以上方法建立的神經網路，我們利用了活體影像和免疫螢光染色檢測其型態與功能完整度，希望未來將可以與微電極陣列結合應用，進而去了解神經網路對於各種形式的訊號輸入所產生的生化或是生理反應。

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