最近幾年，基因序列資訊結合了最新的分子與結構生物學而成為我們現在”結構基因體學”的概念，即是蛋白質的3D結構決定是以整個基因組為範疇。我們常常無法單純從氨基酸序列的組合中去歸納其生物功能，而蛋白質3D結構的相關資訊可以為我們提供一些線索。
幽門螺旋桿菌（Helicobacter pylori, HP）是一個格蘭氏陰性菌、成長慢、具有鞭毛及螺旋外狀的細菌。HP會在人類的胃中生長，幾乎全世界有一半的人口均受此菌感染。而幽門螺旋桿菌感染人類之後的所產生疾病包含有消化性潰瘍、胃黏膜相關的淋巴組織瘤或胃線癌等等。所以我們選擇了幽門螺旋桿菌作為我們整個結構基因體計畫的主角。這篇論文主要是希望使用multi-dimensional NMR的技術，來決定新奇（novel）蛋白質HP0495在溶液中的的3D結構。
從PDB的資料庫中，我們可以發現HP0495和CREB binding protein有 27.397% 的相似度，而且和HP1205有很強的protein-protein interaction，其中HP1205和translation elongation factor EF-Tu有很高的相似度，我們經由核磁共振儀,可以得到此蛋白質之骨架序列光譜標定關係 (使用HNCA, HNCO, HN(CO)CA, HN(CA)CO, CBCANH, and CBCA(CO)NH 光譜，以及selection label光譜). 每個殘基側鏈之氫光譜則是經由TOCSY-HSQC, HCCH-TCOSY, 以及H(CC)(CO)NH光譜實驗所觀測.而 phi 和 psi backbone torsion angles值是使用TALOS database system去預測.我們再經由氫氘交換,NOE光譜的關連性以及 J耦合常數以及化學位移索引之統合分析, 可以將蛋白質的二級結構判斷出來,同時從3D 15N-NOESY-HSQC 和3D 13C-NOESY-HSQC的光譜中,藉由signal intensities來計算proton-proton distances，而得到HP0495大概的結構，希望能進而從結構上的特徵來推測HP0495在生物上所扮演的角色。

In the last few years, genome sequencing projects have produced a wealth of information on the organization of living organisms. The flood of sequence information coupled with recent advances in molecular and structure biology have led to the concept of “structural genomics”, the determination of three-dimensional protein structures on a genome-wide scale. An important use of three-dimensional structural information of proteins is to uncover clues as to a protein’s functions that are not detectable from sequence analysis.
H. pylori is a micro-aerophilic Gram-negative, slow-growing, spiral-shaped and flagellated bacterium that colonizes the stomachs of an estimated half of all humans. (30% of the population in developed and up to 90% of the population in developing countries.) In a subset of humans, H. pylori infection leads to serious disease such as peptic ulcer disease, mucosa-associated lymphoid tissue lymphoma or gastric adenocarcinoma. In this paper, we will use multi-dimensional NMR techniques to determine the three-dimensional solution structures of novel protein(HP0495)
In the PDB database, HP0495 has 27.397% sequence identity with a CREB binding protein. HP0495 have interacts with HP1205 which is strongly similar to translation elongation factor EF-Tu .The backbone sequential assignments were determined from six triple resonance experiments (HNCA, HNCO, HN(CO)CA, HN(CA)CO, CBCANH, and CBCA(CO)NH). Proton side chain assignments were obtained from TOCSY-HSQC, H(CC)(CO)NH, HCCH-TOCSY and HCCH-COSY. And TALOS is a database system for empirical prediction phi and psi backbone torsion angles using a combination of five kinds (HA, CA, CB, CO, N) of chemical shift assignments. Secondary structures were identified via NOE correlations, amide exchange, J coupling constants, and CSI analysis. Proton-proton distances can be estimated from the signal intensities in the 15N-NOESY-HSQC and 13C-NOESY-HSQC spectra to calculate structures.
We hope to use of three-dimensional structure of HP0495 to uncover clues of it's functions。

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