本篇論文在闡述利用耐輻射奇異球菌Deinococcus radiodurans 靜止期無菌培養基(cell free broth)來產生金屬銀奈米線。由實驗結果知：D. radiodurans 靜止期CFB 所產生金屬銀奈米線之產量(約2.5 x 105 wires / mL)與平均長度(約62.5 ±9.1 µm)，均優於含菌菌液所生成之金屬銀奈米線(產量約3.5 x 103 wires / mL, 平均長度約38.3 ± 2.3 µm)。而D. radiodurans 生合成金屬銀奈米銀線能力，與其所處生長時期有關。由對數生長時期將轉入靜止期時，方始有銀線產生。但是此金屬銀奈米線生成能力可以利用加入0.01 ％ (v/v)之靜止期CFB 誘發出來。此外，CFB 之生成金屬銀奈米線能力對熱穩定，但在銀奈米線生成的反應過程中卻會受熱破壞；酸鹼值需為中性才能生成金屬銀奈米線。CFB 系統除了提供大量金屬銀奈米線，利於未來研究所需。透過了解影響該系統產生金屬銀奈米線的因子，更可以近一步了解D. radiodurans 細胞與細胞間的交互作用。

In this thesis, we use the CFB (cell free broth) of Deinococcus radiodurans stationary phase culture as a system to fabricate silver nanowire. After series of
experiments, we found that the amount of silver nanowires fabricated by D. radiodurans CFB (with 2.5 x 105 wires / mL, average length is about 62.5 ± 9.1 µm) are better than those fabricated by cell culture (with 3.5 x 103 wires / mL, average length is about 38.3 ± 2.3 µm). And the ability to fabricate silver nanowire is growth-phase dependent, emerging at late-log phase, and optimizing at stationary phase. Furthermore, the ability to fabricate silver nanowire could be induced by CFB ( 0.01％, v/v ). Besides that, we also found the CFB is heat-tolerance. But the reaction of silver nanowire synthesis is not heat stable, and must react under neutral pH. In conclusion, using CFB system to fabricate silver nanowire, could provide more silver nanowires for further research and development. And by studying the factors which influence the silver fabricated ability of CFB, could be a way to know more about cell-to-cell interaction of D. radiodurans.

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