神經軸突是由神經元細胞本體延伸而出的細長神經纖維，其在發育階段及神經系統的功能上扮演著不同及重要的角色。本研究發展出一套玻璃基板的裝置，藉以用來生產大量的軸突蛋白以提供做蛋白質與RNA的分析。在玻璃基板的表面上培養大鼠海馬迴的神經細胞，大量的軸突有別於細胞本體貼附的區域，可被分離引導生長至不同的區域。經由免疫螢光染色的研究結果顯示，這些引導軸突生長的區域惟獨只有軸突生長貼附在上，且未有其他樹突結構纖維或神經膠細胞的存在，因此神經軸突在此玻璃基板上可被確實的分離出來，同時可經由自製切割器配合基板後面所設計之線性刻痕，進行玻片切斷與收集次細胞單位樣本的動作。利用一維、二維電泳凝膠法及西方點墨等分析法來研究揭露神經軸突和細胞比較有何不同的蛋白組成，而RT-PCR的分析結果也指出神經軸突局部內含有RNAs的存在。
上述神經細胞培養基板經由變化修飾後，也可作為評估不同分子對軸突的生長速率及軸突分化成前突觸之能力的定量分析。結果指出，laminin及neuroligin-1能增加軸突的生長，collagen則無明顯增加作用；然而只有neuroligin-1能促進軸突分化成前突觸，laminin和collagen則無觀察到有增加作用的結果。此外，此基板裝置可經由簡易的修飾變化進行其他神經軸突相關的研究，例如軸突結構之分子組成，包括有軸突柄和生長錐等，以及軸突損傷之退化與再生的過程。經由這些多方不同的運用，此基板裝置將可做為一種研究軸突功能蛋白之有力實用的技術平台。

鄭佳和 (2009) 結合微接觸壓印與模版侷限收集分析海馬迴神精軸突與生長錐蛋白質。國立清華大學生命科學系碩士論文

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