螢光探針在科學及醫學上是一項重要的工具。目前，螢光探針技術，已應用於許多不同蛋白質的偵測方法，例如，籠閉型螢光探針、生物正交化學探針及其他蛋白質標記技術。螢光探針有著操作方便、高靈敏度以及高專一性等優點，但如果探針保存不易，則在探針的應用上將會大打折扣。在本篇論文中，利用特戊酸提供的立體障礙，發展出具有高穩定性及選擇性的親和型螢光標記探針(Affinity-based probe)，以選擇性標記目標蛋白hCA II。此探針成功應用於細胞影像中蛋白質的標記偵測，且只要修改螢光基團或是配體，即可利用不同的螢光來偵測不同的目標蛋白。我們相信此新型的蛋白質標記探針未來可應用在藥物發現、疾病診斷與基礎生物化學研究上。

Fluorescent probe is a powerful technique in biochemical research. In the past decades, many fluorescent probes have been developed for protein detection such as caged-probes, protein tag technique, and bioorthogonal chemistry reaction probes. However, there are some limitations in these techniques, such as requiring genetic modification, gradual degradation in stock solutions. In this research, we improve the stability of reactive electrophile phenyl ester with large steric hindrance pivalate group. The probe was applied to label the target protein in cell lysates and in live cells to detect intracellular protein. We believe that this novel protein labeling probe design can be applied in many other applications, such as drug discovery, medical diagnosis, and fundamental biochemical studies.

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