非侵略性公鼠的理毛行為探討｜國立清華大學博碩士論文庫

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研究生：	蘇鈺珊 Su, Yu-Shan
論文名稱：	非侵略性公鼠的理毛行為探討 Study of Allogrooming in Non-aggressive Male Mice
指導教授：	郭崇涵 Kuo, Tsung-Han
口試委員:	焦傳金 Chiao, Chuan-Chin 汪宏達 Wang, Horng-Dar
學位類別：	碩士 Master
系所名稱：	生命科學暨醫學院 - 系統神經科學研究所 Institute of Systems Neuroscience
論文出版年：	2020
畢業學年度：	108
語文別：	英文
論文頁數：	47
中文關鍵詞：	相互理毛行為、利他主義行為、社交行為、居住-入侵者實驗、社會階級
外文關鍵詞：	social behaviors, allogrooming, altruistic behavior, resident-intruder assay, social hierarchy
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動物為了生存與繁衍，演化出了多樣的社交行為，透過探討動物社交行為
的意涵，可以作為了解人類社交互動的依據。在眾多的社交行為中，相互理毛
行為是一種廣為人知的社交互動，從昆蟲、鳥類到哺乳類都有該行為的記載，
在研究中許多人也相信互相理毛行為在社交互動中扮演著一種促進正向連結的
功能。甚至許多人將理毛行為視為一種利他主義行為(altruistic behavior)，
因為理毛者必須付出精力來幫助被理毛者。在本篇研究中，我們利用侵入者實驗去
探討不具侵略性的小鼠的社會行為，發現社會階級較低與嗅覺缺失的小鼠都明
顯地增加相互理毛行為。同時我們也發現在受到觸覺的刺激下，像是在入侵小
鼠身上塗抹礦物油或是口紅膠，可以誘導更多的互相理毛行為產生。除此之
外，我們也證實互相理毛可以對接受者起到清潔與舒緩壓力的效果。最後我們
探討互相理毛行為背後的神經機制發現破壞杏仁核可以有效的增加相互理毛行
為。本研究對於小鼠相互理毛提供了新的知識，並在未來提供一個新的機會去
探討相互理毛行為所涉及的神經迴路以及背後的生物意義。

Animals perform various kinds of social behaviors that are crucial to their survival and reproduction. Studies of these behaviors in animals provide us important information related to social interaction in human. Allogrooming is a well-known social behavior and has been reported in multiple species from insects, birds, to
mammals. Previous study generally recognized that allogrooming acts as an altruistic behavior because groomers paid the cost to benefit recipients and therefore functions as a positive connection during social interaction. In this study, we examined social behaviors in non-aggressive male mice under resident-intruder assay and found
increase of allogrooming in both subordinate and anosmic mice. Meanwhile, tactile stimuli such as cover exotic material could further induce more allogrooming. In addition, we confirmed that the observed allogrooming in our study is an altruistic behavior by cleaning and calming intruders. Lastly, our data showed that posterior dorsal subdivision of medial amygdala can regulate allogrooming. Our studies provided new information about mouse allogrooming and presented a novel platform for exploring underlying neural circuits and biological meaning in future research.

Table of content
Introduction....................................................6
1. Social behaviors in animals ...............................6
2. Allogrooming in different species .........................6
3. Allogrooming in mice ......................................7
4. Aggression in mice.........................................7
5. Research aim...............................................8
Materials and method............................................10
1. Animals ...................................................10
2. Ablation of the main olfactory epithelium .................10
3. Establishing and identifying social hierarchy .............10
4. Mice castration surgery ...................................10
5. Standard resident-intruder assay ..........................11
6. Evaluating of poster color cleaned efficiency .............11
7. Changing intruder’s fur by covered foreign material assay..11
8. Two-choice assay ..........................................12
9. Blood collection and corticosterone level measurement .....12
10. Stereotaxic surgery ......................................12
11. Prepared for brain tissue and immunohistochemistry .......13
12. Statistics ...............................................14
Results .......................................................15
1. Elimination of aggression on male mice.....................15
1.1. Subordinate males performed allogrooming.................15
1.2. Ablation of MOE induces allgrooming......................15
1.3. Castration eliminated both allogrooming and aggression...16
1.4. Somatosansation induced allogrooming.....................17
1.5. Allogrooming cleans recipients’ fur .....................17
1.6. Allogrooming could calm intruder.........................18
2. Mechanism of allogrooming .................................19
2.1. MeApd lesion promoted allogrooming behavior .............19
Discussion ....................................................20
1. Allogrooming could observed in non-aggression male mice ...20
2. Allogrooming had hygienic function ........................21
3. Allogrooming made more fitness to groomee .................22
4. MeApd lesion modulated both aggression and allogrooming ...22
5. Conclusion ................................................23
Reference .....................................................24
Figure ........................................................27
                                

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