近年來核射線在分子醫學的臨床應用發展迅速，本研究針對用放射性核素來打斷DNA的實驗數據，提出一機率模型做資料分析。分析的實驗數據是由放射性鎝(99mTc)進入DNA後，用光鑷子技術(optical tweezers)觀測DNA斷裂的時間。我們根據DNA斷裂行為假設各種參數，以建立描述此DNA斷裂現象的機率模型。最後藉由兩種方法：最大擬概似估計法(Maximum Pseudo Likelihood Estimation (MPLE))和動差法(MME)估計放射能量將DNA打斷的機率，並比較兩種方法，以及探討參數對估計上的影響。

[1] Schwochau K. (2000). Technetium-Chemistry and Radiopharmaceutial Applications, Wiley-VCH, Weinheim.
[2] Sampson C. B. (1999). Textbook of Radiopharmacy, 3rded., Gordon and Breach, Amsterdam.
[3] Behr T. M., Sharkey R. M., Juweid M. E., Dunn R. M., Vagg R. C., Ying Z. L., Zhang C. H., Swayne L. C., Vardi Y., Siegel J. A. and Goldenberg D. M. (1997). “Phase I / II Clinical Radioimmunotherapy with an Iodine-131-Labele Anti-Carcinoembryonic Antigen Murine Monoclonal Antibody IgG”, The Journal of Nuclear Medicine, 38, 858-870.
[4] Sastry K. S. R. (1992). “Biological effects of the Auger emitter iodine-125: A review. Report No.1 of AAPM Nuclear Medicine Task Group No. 6”, MedicalPhysics, 19, 1361-1370.
[5] Humm J. L. and Charlton D. E. (1989). “A new calculational method to assess the therapeutic potential of Auger electron emission”, International Journal Radiation Oncology Biology Physics, 17, 351-360.
[6] Ftacnikova S. and Bohm R. (2000). “Monte Carlo Calculations of Energy Deposition in DNA for Auger Emitters”, Radiation Protection Dosimetry, 92, 269-278.
[7] Hofer K. G. (1998). “Dosimetry and Biological Effects of Incorporated Auger Emitters”, Radiation Protection Dosimetry, 79, 405-410.
[8] Hofer K. G. (2000). “Biophysical Aspects of Auger Processes”, Acta Oncologica, 39, 651-657.
[9] Donoghue J. A. O. and Wheldon T. E. (1996). “Targeted radiotherapy using Auger electron emitters”, Physics in Medicine and Biology, 41, 1973-1992.

[10] Lang M. J., Asbury C. L., Shaevitz J. W. and Block S. M. (2002). “An automated two-dimensional optical force clamp for single molecule studies”. Biophysical Journal Volume, 83, 491-501.
[11] Ross S. M. (2003), Introduction to Probability Models, 8thed., 288-288.
[12] 蕭珮芬 (2007), DNA雙股斷裂的機率模型, 國立清華大學統計研究所碩
士論文.