從美國腎臟登錄系統(USRDS)在2014的年度報告中，顯示台灣在罹患腎衰竭末期的人口比例位居世界第一，而且人數仍然有逐年增加的趨勢。雖然現今醫療上有部分治療腎損傷的方法，不過這些治療方式一般都會產生某些副作用，不論對於醫生或病人來說是都是非常棘手的疾病，若我們可以在腎臟疾病的早期就診斷出問題，就能及早預防，而近年生物標記物NGAL(neutrophil gelatinase-associated lipocalin)被認為能應用於早期診斷急性腎損傷，對該症有極佳的預測能力。本研究使用銀奈米線，設計製作以銀奈米線為基礎的微電極電子式生醫感測器，運用於檢測急性腎損傷生物標記物NGAL，並且本研究利用銀奈米線反應靈敏之特性，加上奈米線表面化學修飾急性腎損傷標記物生物辨識層，配合串聯式與並聯式電極增加靈敏度，達到能準確檢測生物標記物之目的。本研究實驗中，利用了介電泳的原理配合微流體進行奈米線組裝，通過控制不同參數來達成穩健組裝單根銀奈米線。通過實驗確認，徹底探討流道高度、溶液濃度與流體速度等製成參數最佳化。而同時也透過傅立葉轉換紅外線光譜儀分析下，在修飾後銀奈米線上分析出o-PD(o-phenylenediamine)峰值存在，以及醯胺等蛋白質特徵波段有峰值發生，確認單體修飾成功。
本研究應用於急性腎損傷生物標記物NGAL檢測，以串聯與並聯電極晶片進行檢測，其中最優者為並聯式三對電極晶片，靈敏度可達0.25 pF/ppb，LOD(limit of detection)為10 ppb。在拓印因子測試上來看，MIP(molecularly imprinted polymer)生醫感測器有顯著的性能提升，MIP/NIP(non-imprinted polymer)為537%。選擇性的測試方面，分別使用CRP(C-Reactive protein) PBS溶液、IL-6 (interleukin-6) PBS溶液與BSA(Bovine serum albumin)水溶液進行生醫感測器的選擇性測試，實驗結果顯示本研究對NGAL蛋白質具有優秀的選擇性，電子式響應變化比例分別為其他三種蛋白質的4.47倍、4.91倍與7.5倍。

In the 2014 annual report of The United States Renal Data System (USRDS), Taiwan's population at the end of kidney failure is ranked first in the world, and the number is still increasing year by year. Although there are some methods for treating kidney damage in medical care today, these treatments generally have some side effects, which are very difficult diseases for doctors or patients. If we can diagnose problems early in kidney disease, it can be prevented. In recent years, the biomarker NGAL(neutrophil gelatinase-associated lipocalin) is considered to be useful for the early diagnosis of acute kidney injury, and has excellent predictive power for the disease. In this study, a silver nanowire-based microelectrode electronic biomedical sensor was designed and used to detect the acute kidney injury biomarker NGAL.The characteristics, together with the nanowire surface chemical modification of the acute kidney injury marker bio-identification layer, combined with the series and parallel electrodes to increase the sensitivity, to achieve the purpose of accurate detection of biomarkers. In this research experiment, the principle of dielectrophoresis was also used with microfluidics for nanowire assembly, and a single silvery nanowire was assembled by controlling different parameters. Through experiments, it is confirmed that the parameters such as the flow path height, the solution concentration and the fluid velocity are thoroughly optimized. At the same time, through the analysis of Fourier transform infrared spectrometer, the presence of o-PD peaks was analyzed on the modified silver nanowire, and the peaks of protein characteristic bands such as indoleamine occurred, confirming the success of monomer modification.
This study was applied to the detection of acute kidney injury biomarker NGAL, which was tested in series and parallel electrode wafers. The best one was a parallel three-pair electrode wafer with sensitivity of 0.25 pF/ppb and LOD of 10 ppb. In terms of the rubbing factor test, the MIP biomedical sensor has a significant performance improvement with a MIP/NIP of 537%. For selective testing, CRP PBS solution, IL-6 PBS solution and BSA aqueous solution were used for biomedical sensor selectivity test. The results showed that the study has excellent selectivity for NGAL protein. The proportion of electronic response changes was 4.47 times, 4.91 times and 7.5 times that of the other three proteins, respectively.

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