摘要
癌症目前仍是嚴重威脅人類性命的疾病，在 2012 年仍有近千萬的人死於癌症，因此積極發展抗癌症的藥物是必要的。傳統化療藥物雖然可在初期有效抑制癌症細胞，然而它是一個不受控制的藥物，因為正常的細胞也會受到影響，更嚴重的事是在癌症後期，癌細胞對化療藥物將具有抗藥性，而正常細胞仍然沒有抵抗力，因此尋找下一個能有效針對癌細胞的藥物成為世界重要的議題。近幾年細菌素被發現不僅具有抗菌的功能，甚至可作用於部分癌細胞，其中有幾個細菌素更展現出高度專一性。
本實驗藉由修改前人在 Lactobacillus Casei 菌株中找到的 Class II 細菌素 BD21，並利用基因轉殖技術殖入大腸桿菌且大量表現，之後進行細胞活性測試，由此實驗發現經修改後的細菌素 N3 可區別腸癌細胞與正常細胞。

Abstract
Cancer is the most dangerous disease in the world, despite their have a lot of treatment, however cancer doesn’t disappear from human. In the traditional chemotherapeutic agents which used for treating
cancer not only have no specificity on normal cell, but also it will have a lot of side effect, even the cancer cell will develop resistant ability. Hence, human need to discover novel anti-cancer drugs against different types of cancer. In the recent research have pointed out the therapeutic potential drugs, various bacteriocins.
In this thesis we use BD21whcih bacteriocin belongs to class II and have anticancer ability as model to exchange its amino acids for pursuing more higher cytotoxicity to colon cancer. We found a protein that just need half concentration to kill colon cancer.

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