磷酸鹽是生物體內重要的營養鹽。為了從相對濃度較低的土壤中獲取磷酸鹽類，植物演化出不同種的運送蛋白。其中一大類為SXP結構域的蛋白。含有SXP結構域的蛋白質可分為數種：SXP結構域單獨構成、SXP結構域和RING結構域、SXP結構域和MFS結構域、以及SXP結構域和EXS結構域等等。在這次的研究中，我們嘗試表現下列蛋白：屬於SPX-RING的AtNLA、屬於SPX-MFS的AtSPMF1、以及屬於SPX-EXS中的PHO家族內的AtPHO1和OsPHO1;2。在我們的研究中，AtNLA、AtPHO1各自分為兩段表現，而AtSPMF1、OsPHO1;2則是維持全段基因。目前已經成功地把AtSPMF1以及OsPHO1;2建構至載體pYESII。

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