本研究利用Cu(II)與20種自然胺基酸與β-Ala 形成的銅錯合物，於25°C的磷酸鹽溶液中活化過氧化氫，並偵測反應中2-甲喹啉藍(QB)的初始氧化速率來探討銅錯合物活化過氧化氫效率。爲了確認銅物種的影響，實驗中探討許多參數:pH、離子強度、溫度以及Cu(II)、胺基酸和過氧化氫的濃度。QB的氧化速率隨銅胺基酸錯合物濃度成一級反應，隨過氧化氫濃度成多級反應，這符合Michaelis-Menten動力方程式的描述。在20種胺基酸與β-Ala中，Cu-His錯合物有最強的活性。不同胺基酸的催化活性差異可達500倍，而此差異可能是由於胺基酸的結構與官能基影響過氧錯合物的形成。由實驗中發現1:1結合的銅胺基酸錯合物(CuL)是主要的活性催化物。最後藉由一價銅與兩價銅錯合物的實驗結果以及產物分析，推導出過氧化氫活化的反應機制。

Activation of hydrogen peroxide by Cu(II) complexes of 20 native amino acids (L) and β-Ala has been studied by measuring the initial oxidation rate of quinaldine blue(QB) in aqueous phosphate media at 25°C. System parameters such as pH, ionic strength, temperature and concentrations of Cu(II), amino acids, and hydrogen peroxide are examined to characterize the effects of Cu(II) speciation. The oxidation rate of QB is first order in Cu(II)-amino acid complexes and variable order in hydrogen peroxide, which can be explained in terms of Michaelis-Menten kinetics. Among 20 native amino acids and β-Ala, Cu(II)-histidine complex could more easily activate hydrogen peroxide than any other Cu(II)-amino acid complexes. The catalytic activities vary by about 500-fold and are discussed in terms of the ligand structure and function group, which affects the formation of lignad-Cu(II)-peroxide complexes. The catalytic activities of Cu(II) complexes with all 20 amino acids demonstrate that the 1:1 Cu(II) complexes (CuL) are the dominant species forming the active copper complex catalyst. Based on the experimental results of Cu(I) and Cu(II) complex systems and product measurement, a peroxide activation mechanism is proposed .

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